| 1 | Mutation profile and its correlation with clinicopathology in Chinese hepatocellular carcinoma patients显示文摘Background:Hepatocellular carcinoma(HCC)is one of the most common causes of cancer worldwide.Although many studies have focused on oncogene characteristics,the genomic landscape of Chinese HCC patients has not been fully clarified.Methods:A total of 165 HCC patients,including 146 males and 19 females,were enrolled.The median age was 55 years(range,27-78 years).Corresponding clinical and pathological information was collected for further analysis.A total of 168 tumor tissues from these patients were selected for next-generation sequencing(NGS)-based 450 panel gene sequencing.Genomic alterations including single nucleotide variations(SNV),short and long insertions and deletions(InDels),copy number variations,and gene rearrangements were analyzed.Tumor mutational burden(TMB)was measured by an algorithm developed in-house.The top quartile of HCC was classified as TMB high.Results:A total of 1,004 genomic alterations were detected from 258 genes in 168 HCC tissues.TMB values were identified in 160 HCC specimens,with a median TMB of 5.4 Muts/Mb(range,0-28.4 Muts/Mb)and a 75%TMB of 7.7 Muts/Mb.The most commonly mutated genes were TP53,TERT,CTNNB1,AXIN1,RB1,TSC2,CCND1,ARID1A,and FGF19.SNV was the most common mutation type and C:G>T:A and guanine transformation were the most common SNVs.Compared to wild-type patients,the proportion of Edmondson grade III-IV and microvascular invasion was significantly higher in TP53 mutated patients(P<0.05).The proportion of tumors invading the hepatic capsule was significantly higher in TERT mutated patients(P<0.05).The proportion of Edmondson grade I-II,alpha fetoprotein(AFP)<25μmg/L,and those without a history of hepatitis B was significantly higher in CTNNB1 mutated patients(P<0.05).CTNNB1 mutations were associated with TMB high in HCC patients(P<0.05).Based on correlation analysis,the mutation of TP53 was independently correlated with microvascular invasion(P=0.002,OR=3.096)and Edmondson grade III-IV(P=0.008,OR=2.613).The mutation of TERT was independently correlated with tumor invasion of the liver capsule(P=0.001,OR=3.030),and the mutation of CTNNB1 was independently correlated with AFP(<25μmg/L)(P=0.009,OR=3.414).Conclusions:The most frequently mutated genes of HCC patients in China were TP53,TERT,and CTNNB1,which mainly lead to the occurrence and development of HCC by regulating the P53 pathway,Wnt pathway,and telomere repair pathway.There were more patients with microvascular invasion and Edmondson III-IV grade in TP53 mutated patients and more patients with hepatic capsule invasion in TERT mutated patients,while in CTNNB1 mutated patients,there were more patients with Edmondson I-II grade,AFP<25μmg/L,and a non-hepatitis B background.Also,the TMB values were significantly higher in CTNNB1 mutated patients than in wild type patients. | Shuo Wang Huasheng Shi Tao Liu Manjiang Li Sanshun Zhou Xuan Qiu Zusen Wang Weiyu Hu Weidong Guo Xiaoqian Chen Honglin Guo Xiaoliang Shi Junping Shi Yunjin Zang Jingyu Cao Liqun Wu | 2021 | Hepatobiliary Surgery and Nutrition2021，10，2： | 8 |
| 3 | Basic deep eutectic solvents as reactant, template and solvents for ultra-fast preparation of transition metal oxide nanomaterials显示文摘Developing a new type of deep eutectic solvents(DESs)is indispensable for expanding their application in various fields.Here,we report a series of new highly basic DESs.FT-IR,quantitative 1 H NMR,MD simulation and physical properties show that these basic liquids are made up of hydroxide acceptor of alkali metal hydroxides in which the hydrogen bonding interactions coordinate the donor.These DESs can be played three roles as new solvents,template and reactant for facile and ultra-fast preparation of transition metal oxide nanomaterials such as NiCo2 O4,MnCo2 O4,NiMn2 O4,CoCu2 O4 and Co3 O4 under mild condition.This work shows one of the low energy-intensive methods for nanomaterial preparation.These initial findings of basic deep eutectic solvents provide a potential applicability around the systematic development of transition metal oxide nanosheets. | Jia Chen Mohammad Chand Ali Ruirui Liu Jean Claude Munyemana Zhan Li Honglin Zhai Hongdeng Qiu | 2020 | Chinese Chemical Letters2020，31，6： | 2 |
| 5 | Research on cross-chain and interoperability for blockchain system显示文摘At present, there is an urgent need for blockchain interoperability technology to realize interconnection between various blockchains, data communication and value transfer between blockchains, so as to break the ‘value silo’ phenomenon of each blockchain. Firstly, it lists what people understand about the concept of interoperability. Secondly, it gives the key technical issues of cross-chain, including cross-chain mechanism, interoperability, eventual consistency, and universality. Then, the implementation of each cross-chain key technology is analyzed, including Hash-locking, two-way peg, notary schemes, relay chain scheme, cross-chain protocol, and global identity system. Immediately after that, five typical cross-chain systems are introduced and comparative analysis is made. In addition, two examples of cross-chain programmability and their analysis are given. Finally, the current state of cross-chain technology is summarized from two aspects: key technology implementation and cross-chain application enforcement. The cross-chain technology as a whole has formed a centralized fixed mechanism, as well as a trend of modular design, and some of the solutions to mature applications were established in the relevant standards organizations, and the cross-chain technology architecture tends to be unified, which is expected to accelerate the evolution of the open cross-chain network that supports the real needs of the interconnection of all chains. | Li Ming Qiu Honglin Xu Quanqing Song Wenpeng Liu Baixiang | 2021 | The Journal of China Universities of Posts and Telecommunications2021，28，5： | 0 |
| 6 | Engineering a customized nanodrug delivery system at the cellular level for targeted cancer therapy显示文摘Drug administration customized to individual cells could intrinsically address cancer heterogeneity and provide a safe and effective method for delivering personalized treatment. To accomplish this, we developed a smart nanodrug delivery system characterized by cancer cell-targeted drug delivery and intracellular biomarker-responsive drug activation. This system was composed of a long-nicked DNA duplex formed by tandem hybridization of two extended antisense oligonucleotides whose ends were separately blocked with a cancer cell-specific aptamer, AS1411,and a replaceable anti-biomarker probe(ABP). We demonstrated that this DNA nanodrug was directed to cancer cells with the guidance power of AS 1411 and then activated by the presence of a given intracellular biomarker. By using such a belt-and-braces strategy, this DNA nanodrug system could safely and efficiently accelerate apoptosis of target cancer cells. Moreover, since the expression level of biomarkers tends to indicate the specific physiological state of individual cells, biomarker-responsive activation of the nanodrug is expected to enable customized drug administration at the cellular level. | Jin Li Liping Qiu Sitao Xie Jing Zhang Liqin Zhang Honglin Liu Juan Li Xiaobing Zhang Weihong Tan | 2018 | Science China Chemistry2018，61，4： | 0 |
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