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| 1 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 2 | Role of different childbirth strategies on pelvic organ prolapse and stress urinary incontinence: a prospective study显示文摘背景:筋膜、肌肉发达的支持结构的创伤的损坏可以在分娩期间是压力尿失禁(穗) 和骨盆的机关脱垂(流行音乐) 的发展的一个主要因素。这研究的目的是有希望地在流行音乐和穗上调查不同分娩策略的角色。方法:120 个选择怀孕女人的一个总数被招募并且把组划分了成二:阴道交货组(n = 72 ) 并且选择恺撒的交货组(n = 48 ) 。这些女人与穗表被询问并且在 6 以内与流行音乐数量化离子(POP-Q ) 估计了 - 在交货以后的 8 个星期。穗的关联和有交货的策略的 POP-Q 的结果被分析。t, chi (2 ) 测试,和 Mann-Witney 测试被用于统计分析。结果:在 120 个女人之中,穗在 29 期间被开发 - 在 20 个病人(16.6%) 的 30 个妊娠的星期,谁(70%) 14 有穗的症状到 6 个星期为止产后。完全 20 (16.6%) 女人们在交货以后有穗症状。产后的穗的流行断然在 29 期间与穗的出现被相关 - 30 个妊娠的星期(P <
0.001 ) 。在阴道交货组,承受的女人的 100% 形成 6 个星期产后的尿脱垂,当 87.5% 那些在选择恺撒的交货组织发达流行音乐时(P <
0.01 ) 。在阴道交货组的一度、二度的尿脱垂的百分比分别地是 20.8% 和 79.2% ,它与在选择恺撒的交货组的那些显著地不同(64.6% 和 22.9% ;两 P = 0.000 ) 。结论:怀孕和交货在穗和流行音乐的发展起一个病因学的作用。穗的发作在迟了的怀孕期间暗示产后的穗症状的重要风险。流行音乐的流行比在恺撒的交货以后在阴道交货以后是显著地更高的。 | ZHU Lan BIAN Xu-ming LONG Yan LANG Jing-he | 2008 | Chinese Medical Journal2008，，3： | 18 |
| 3 | Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing显示文摘Development of tools for targeted modifications of specific DNA sequences in plants is of great importance to basic plant biology research as well as crop improvement.The ability to cut DNA at specific locations in the genome to generate doublestrand breaks(DSBs)in vivo is a prerequisite for any genome editing efforts.Several engineered nucleases including zinc finger nucleases(ZFNs)and transcriptional activator-like nucleases(TALENs)have been successfully used to edit genomes.However, | Yubing He Tao Zhang Ning Yang Meilian Xu Lang Yan Lihao Wang Rongchen Wang Yunde Zhao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 4 | The Qitai radio telescope显示文摘This study presents a general outline of the Qitai radio telescope(QTT)project.Qitai,the site of the telescope,is a county of Xinjiang Uygur Autonomous Region of China,located in the east Tianshan Mountains at an elevation of about 1800 m.The QTT is a fully steerable,Gregorian-type telescope with a standard parabolic main reflector of 110 m diameter.The QTT has adopted an umbrella support,homology-symmetric lightweight design.The main reflector is active so that the deformation caused by gravity can be corrected.The structural design aims to ultimately allow high-sensitivity observations from 150 MHz up to115 GHz.To satisfy the requirements for early scientific goals,the QTTwill be equipped with ultra-wideband receivers and large field-of-view multi-beam receivers.A multi-function signal-processing system based on RFSo C and GPU processor chips will be developed.These will enable the QTT to operate in pulsar,spectral line,continuum and Very Long Baseline Interferometer(VLBI)observing modes.Electromagnetic compatibility(EMC)and radio frequency interference(RFI)control techniques are adopted throughout the system design.The QTT will form a world-class observational platform for the detection of lowfrequency(nano Hertz)gravitational waves through pulsar timing array(PTA)techniques,pulsar surveys,the discovery of binary black-hole systems,and exploring dark matter and the origin of life in the universe.The QTT will also play an important role in improving the Chinese and international VLBI networks,allowing high-sensitivity and high-resolution observations of the nuclei of distant galaxies and gravitational lensing systems.Deep astrometric observations will also contribute to improving the accuracy of the celestial reference frame.Potentially,the QTT will be able to support future space activities such as planetary exploration in the solar system and to contribute to the search for extraterrestrial intelligence. | Na Wang Qian Xu Jun Ma Zhiyong Liu Qi Liu Hailong Zhang Xin Pei Maozheng Chen Richard N.Manchester Kejia Lee Xingwu Zheng Hans J.Kärcher Wulin Zhao Hongwei Li Dongwei Li Martin Süss Matthias Reichert Zhongyi Zhu Congsi Wang Mingshuai Li Rui Li Ning Li Guljaina Kazezkhan Wenming Yan Gang Wu Lang Cui Ming Zhang Haitao Li | 2023 | Science China(Physics,Mechanics & Astronomy)2023，66，8： | 14 |
| 5 | Proportion of Uterine Malignant Tumors in Patients with Laparoscopic Myomectomy: A National Multicenter Study in China显示文摘 | Hua Yang Xiao-Chuan Li Chen Yao Jing-He Lang Hang-Mei Jin Ming-Rong Xi Gang Wang Lu-Wen Wang Min Hao Yan Ding Jie Chen Jian-Qing Zhang Lu Han Cheng-Xiu Guo Xiang Xue Yan Li Jian-Hua Zheng Man-Hua Cui Huai-Fang Li Guang-Shi Tao Long Chen Su-Min Wang An-Wei LU Ze-Hua Huang Qing Liu Ya-Li Zhuang Xiang-Hua Huang Gen-Hai Zhu Ou-Ping Huang Li-Na Hu Mu-Jun Li Hong-Lin Zhou Jing-Hui Song Lan Zhu | 2017 | Chinese Medical Journal2017，，22： | 10 |
| 6 | Architecture Optimization of More Electric Aircraft Actuation System显示文摘在飞机的力量来源和致动器的可选的类型由于在更电的技术的快开发是越来越多样的,它做不同力量来源的联合,致动器在在空中的使活动系统的体系结构优化进程变得极其复杂。传统的尝试方法不能满足设计要求。第一,在这糊更多的电的飞机(MEA ) 的作文飞行控制使活动系统(FCAS ) 被介绍,并且可能的建筑学数量是计算的。第二,关于安全可靠性,重量和效率的 FCAS 建筑学的评估标准被建议,并且评估标准值在每控制表面采用的情况中是计算的一样的致动器配置。最后, MEA FCAS 建筑学的优化结果被使用基因算法(GA ) 获得。比作传统的使活动系统建筑学,它仅仅采用伺服阀门控制了水力的致动器,优化更电的使活动系统建筑学的重量能被 6% 减少,并且效率能被 30% 基于安全可靠性要求改进。 | QI Haitao FU Yongling QI Xiaoye LANG Yan | 2011 | Chinese Journal of Aeronautics2011，24，4： | 9 |
| 7 | High-efficiency and multiplex adenine base editing in plants using new TadA variants显示文摘Recently reported adenine base editors(ABEs)exhibit powerful potential for targeted gene correction as well as developing gain-of-function mutants and novel germplasms for both gene function studies and crop breeding.However,editing efficiency varies significantly among different target sites.Here,we investigated the activities of three evolved E.coli adenosine deaminase TadA variants(TadA8e,TadA8.17,and TadA8.20)side-by-side in transgenic rice.We found that TadA8e outperforms TadA8.17 and TadA8.20,and induces efficient A-to-G conversion at all tested sites in the rice genome,including those that were un-editable by ABE7.10 in our previous experiments.Furthermore,V82S/Q154R mutations were incorporated into TadA8e,resulting in a new variant that we named TadA9.Our data show that TadA9 is broadly compatible with CRISPR/SpCas9,CRISPR/SpCas9-NG,and CRISPR/SpRY,as well as CRISPR/ScCas9 nickase systems,achieving comparable or enhanced editing in a larger editing window at diverse PAM sites as compared with TadA8e.Finally,TadA9 was used to simultaneously install novel SNPs in four endogenous herbicide target genes in the commercial rice cultivar Nangeng 46 for potential field application in.weed control.Collectively,we successfully generated a series of novel ABEs that can efficiently edit adenosines in the rice genome.Our findings suggest that TadA9 and TadA8e have great potentials in the development of plant base editors and crop molecular breeding. | Daqi Yan Bin Ren Lang Liu Fang Yan Shaofang Li Guirong Wang Wenxian Sun Xueping Zhou Huanbin Zhou | 2021 | Molecular Plant2021，14，5： | 8 |
| 8 | Icariside Ⅱ inhibits lipopolysaccharide-induced inflammation and amyloid production in rat astrocytes by regulating IKK/IκB/NF-κB/BACE1 signaling pathway显示文摘β-amyloid(Aβ)is one of the inducing factors of astrocytes activation and neuroinflammation,and it is also a crucial factor for the development of Alzheimer’s disease(AD).Icariside II(ICS II)is an active component isolated from a traditional Chinese herb Epimedium,which has shown to attnuate lipopolysaccharide(LPS)-induced neuroinflammation through regulation of NF-κB signaling pathway.In this study we investigated the effects of ICS II on LPS-induced astrocytes activation and Aβaccumulation.Primary rat astrocytes were pretreated with ICS II(5,10,and 20μM)or dexamethasone(DXMS,1μM)for 1 h,thereafter,treated with LPS for another 24 h.We found that ICS II pretreatment dose dependently mitigated the levels of tumor necrosis factor-alpha(TNF-α),interleukin-1 beta(IL-1β),inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2)in the astrocytes.Moreover,ICS II not only exerted the inhibitory effect on LPS-induced IκB-αdegradation and NF-κB activation,but also decreased the levels of Aβ1–40,Aβ1–42,amyloid precursor protein(APP)and beta secretase 1(BACE1)in the astrocytes.Interestingly,molecular docking revealed that ICS II might directly bind to BACE1.It is concluded that ICS II has potential value as a new therapeutic agent to treat neuroinflammation-related diseases,such as AD. | Yong Zheng Yan Deng Jian-mei Gao Chun Lv Ling-hu Lang Jing-shan Shi Chang-yin Yu Qi-hai Gong | 2020 | Acta Pharmacologica Sinica2020，41，2： | 8 |
| 9 | A 14-year multi-institutional collaborative study of Chinese pelvic floor surgical procedures related to pelvic organ prolapse显示文摘Background:It has been a global trend that increasing complications related to pelvic floor surgeries have been reported over time.The current study aimed to outline the development of Chinese pelvic floor surgeries related to pelvic organ prolapse(POP)over the past 14 years and investigate the potential influence of enhanced monitoring conducted by the Chinese Association of Urogynecology since 2011.Methods:A total of 44,594 women with POP who underwent pelvic floor surgeries between October 1,2004 and September 30,2018 were included from 22 tertiary academic medical centers.The data were reported voluntarily and obtained from a database.We compared the proportion of each procedure in the 7 years before and 7 years after September 30,2011.The data were analyzed by performing Z test(one-sided).Results:The number of different procedures during October 1,2011-September 30,2018 was more than twice that during October 1,2004-September 30,2011.Regarding pelvic floor surgeries related to POP,the rate of synthetic mesh procedures increased from 38.1%(5298/13,906)during October 1,2004-September 30,2011 to 46.0%(14,107/30,688)during October 1,2011-September 30,2018,whereas the rate of non-mesh procedures decreased from 61.9%(8608/13,906)to 54.0%(16,581/30,688)(Z=15.53,P<0.001).Regarding synthetic mesh surgeries related to POP,the rates of transvaginal placement of surgical mesh(TVM)procedures decreased from 94.1%(4983/5298)to 82.2%(11,603/14,107)(Z=20.79,P<0.001),but the rate of laparoscopic sacrocolpopexy(LSC)procedures increased from 5.9%(315/5298)to 17.8%(2504/14,107).Conclusions:The rate of synthetic mesh procedures increased while that of non-mesh procedures decreased significantly.The rate of TVM procedures decreased while the rate of LSC procedures increased significantly.Trial registration number:NCT03620565,http://gffzz111276e5ed644ca6scxupocunov6q6vvu.ffgz.tsg.suse.edu.cn. | Zhi-Jing Sun Xiu-Qi Wang Jing-He Lang Tao Xu Yong-Xian Lu Ke-Qin Hua Jin-Song Han Huai-Fang Li Xiao-Wen Tong Ping Wang Jian-Liu Wang Xin Yang Xiang-Hua Huang Pei-Shu Liu Yan-Feng Song Hang-Mei Jin Jing-Yan Xie Lu-Wen Wang Qing-Kai Wu Jian Gong Yan Wang Li-Qun Wang Zhao-Ai Li Hui-Cheng Xu Zhi-Jun Xia Li-Na Gu Qing Liu Lan Zhu | 2021 | Chinese Medical Journal2021，，2： | 7 |
| 10 | Association between the serotonin 1A receptor C(-1019)G polymorphism and major depressive disorder in the northern Han ethnic group in China显示文摘背景最近的研究建议了到主要压抑的混乱(MDD ) 的危险性可能与血清素 1A 受体(5-HTR1A ) 有关 C (-1019)G 多型性。在这研究,我们试图估计在 5-HTR1A C 之间的协会(在北哈特的 -1019)G 多型性和 MDD China.Methods 的种族组 C ( 5-HTR1A 的 -1019)G 与 MDD 和 400 无关的年龄在 400 个病人与聚合酶链反应( PCR )被检测--并且匹配性的健康控制题目。在 C 之间的协会( -1019)G 和 MDD 是统计上在那里的 analyzed.Results 是 MDD 病人之间的统计上重要的差别并且在两个控制遗传型分发( X2=10.913 , df=2 , P=0.004 )并且等位基因频率( X2=10.379 , dr=l , P=0.001 ),并且在遗传型分发和等位基因频率的重要差别在女题目被发现两个(遗传型分发: X2=15.406 , df=2 , P=0.000 ;等位基因频率:X2=15.552, df=l, P=0.000 ) 并且迟了发作的题目(遗传型分发: X2=7.771, df=2, P=0.021;等位基因频率:X2=8.007, df=l, P=0.005 ) 在二 groups.Conclusion,这些结果建议那 5-HTR1A C (-1019)G 多型性可能与 MDD 被联系,它是可能的是为女、迟了发作的 MDD 的易受影响的基因地点。 | WU Ying XU Yong SUN Yan WANG Yan-fang LI Xia LANG Xiao-e WANG Wei-ping ZHANG Ke-rang | 2008 | Chinese Medical Journal2008，，10： | 7 |
| 11 | Intercropping of rice varieties increases the eff iciency of blast control through reduced disease occurrence and variability显示文摘Creating a crop-heterogeneous system by intraspecific mixtures of different rice varieties can substantially reduce blast diseases.Such variety mixtures provide an ecological approach for effective disease control,maintaining high yields with the minimum fungicide applications.Whether such an approach is universally applicable for random rice variety combinations and what is the variation pattern of the diseases under intercropping still remains unclear.We conducted two-year large-scale field experiments involving 47 rice varieties/lines and 98 variety-combinations to compare the occurrence of rice blast in monoculture and intercropping plots at multiple sites.In the experiments,the plant height of the selected traditional varieties was about 30 cm taller,and their life cycle was 10 days longer,than that of the improved rice varieties.The monoculture included either traditional or modern rice varieties grown in separate plots.The intercropping included both traditional and modern rice varieties planted together in the same plots.Results from the field experiments under natural disease conditions demonstrated significant reduction for rice blast disease in intercropping plots,compared with that in monoculture plots.For traditional varieties,the average blast incidence reduced from^26%in monoculture to^10%in intercropping,and the disease severity reduced from^17 in monoculture to^5 in intercropping.For modern varieties,the average blast incidence reduced from^19%in monoculture to^10%in intercropping,and the severity from^10 in monoculture to^4 in intercropping.Traditional rice varieties(~72%)had a much greater increase in the efficiency of disease control than modern varieties(~60%).In addition,substantially lower values of variance in the blast incidence and severity was detected among the variety combinations in intercropping plots than in monoculture plots.Based on these results,we conclude that the intercropping or mixture of rice varieties greatly reduces the occurrence and variation of rice blast disease in particular variety combinations,which makes the intercropping system more stable and consistent for disease suppression on a large scale of rice cultivation. | HAN Guang-yu LANG Jie SUN Yan WANG Yun-yue ZHU You-yong LU Bao-rong | 2016 | Journal of Integrative Agriculture2016，15，4： | 6 |
| 12 | Clinicopathological study on TTV infection in hepatitis of unknown etiology显示文摘AIM: To investigate the state of infection, replication site,pathogenicity and clinical significance of transfusiontransmitted virus (TTV) in patients with hepatitis, especiallyin patients of unknown etiology.METHODS: Liver tissues taken from 136 cases of non-A non-G hepatitis were tested for Tr virus antigen and nucleic acidby in situ hybridization (ISH) and nested-polymerase chainreaction (PCR). Among them, TT virus genome and itscomplemental strand were also detected in 24 cases ofautopsy liver and extrahepatic tissues with ISH. Meanwhile,TTV DNA was detected in the sera of 187 hepatitis patientsby nested-PCR. The pathological and clinical data of thecases infected with nv only were analyzed.RESULTS: In liver, the total positive rate of rrv DNA was32.4 % and the positive signals were located in the nuclei ofhepatocytes. In serus, rrv DNA was detected in 21. 4 %cases of hepatitis A-G, 34.4 % of non-A non-G hepatitis and15 % of healthy donors. The correspondence rate of lrvDNA detection between liver tissue with ISH and sera withPCR was 63.2 % and 89.3 % in the same liver tissues by ISHand by PCR, respectively. Using double-strand probes andsingle-strand probes designed to detect TTV genome, thecorrespondence rate of Trv DNA detected in liver andextrahepatic tissues was 85. 7 %. Using single-strandprobes, TTV genome could he detected in liver andextrahepatic tissues by PCR, but its complemental strands(replication strands) could be observed only in livers. Theliver function of most cases infected with TTV alone wasabnormal and the liver tissues had different pathologicaldamage such es ballooning, acidophilia degeneration,formation of apoptosis bodies and focus of necrosis, but the inflammation in the Iobule and portal area was mild.CONCLUSION: The positive rate of TTV DNA among cases ofhepatitis was higher than that of donors, especially inpatients with nonA non-G hepatitis, but most of them werecoinfected with other hepatitis viruses. Lrv can infect notonly hepatocytes, but also extrahepstic tissues. However,the chief replication place may be liver. The infection of lrvmay have some pathogenicity. Although the pathogenicity iscomparatively weak, it can still damage the liver tissues.The lesions in acute hepatitis (AH) and chronic hepatitis(CH) am mild, but in severe hepatitis (SH) , it can be veryserious and cause liver function failure, therefore, we shouldpay more attention to TTV when studying the possiblepathogens of so-called 'liver hepatitis of unknown etiology'. | Zhong-Jie Hu Zhen-Wei Lang Yu-Sen Zhou Hui-Ping Yan De-Zhuang Huang Wan-Rong Chen Zhao-Xia Luo,Department of Pathology,Beijing You’an Hospital,Beijing 100054,China | 2002 | World Journal of Gastroenterology2002，8，2： | 6 |
| 13 | shRNA-interfering LSD1 inhibits proliferation and invasion of gastric cancer cells via VEGF-C/PI3K/AKT signaling pathway显示文摘BACKGROUND Histone Lysine Specific Demethylase 1(LSD1)is the first histone demethylase to be discovered,which regulates various biological functions by making lysine of histone H3K4,H3K9 and non-histone substrates demethylated.Abnormal regulation of LSD1 is closely related to the occurrence and development of gastric cancer.The change of LSD1 expression level plays an important role in the proliferation and metastasis of gastric cancer cells.The study of its function and mechanism may provide a theoretical basis for early diagnosis and targeted therapy of gastric cancer.AIM To investigate the effect of downregulation of lysine-specific demethylase 1(LSD1)expression on proliferation and invasion of gastric cancer cells and the possible regulatory mechanisms of the VEGF-C/PI3K/AKT signaling pathway.METHODS The LSD1-specific short hairpin RNA(shRNA)interference plasmid was transiently transfected,and expression of LSD1 was downregulated.The cell proliferation ability of LSD1 was observed by CCK-8 assay after downregulating expression of LSD1.Transwell invasion assay was used to observe the change of cell invasion ability after downregulating expression of LSD1.Expression of phosphorylated phosphoinositide 3-kinase(p-PI3K),PI3K,p-AKT,AKT,vascular endothelial growth factor receptor(VEGFR)-3,matrix metalloproteinase(MMP)-2 and MMP-9 in each group was detected by Western blotting.RESULTS The cell proliferation ability of transiently transfected LSD1-shRNA interference plasmid group was significantly lower than that of the control group(P<0.05).Transwell invasion assay showed that the number of cells across the membrane of the LSD1-shRNA transfection group(238.451±5.216)was significantly lower than that of the control group(49.268±6.984)(P<0.01).Western blotting showed that expression level of VEGF-C,p-PI3K,PI3K,p-AKT,AKT,VEGFR-3,MMP-2 and MMP-9 in the LSD1-shRNA group was significantly lower than that in the control group(P<0.05).CONCLUSION Downregulation of LSD1 expression inhibits metastatic potential of gastric cancer cells,and VEGF-C-mediated activation of PI3K/AKT signaling pathway,which may be an important mechanism for inhibiting lymph node metastasis in gastric cancer cells. | Hong-Ming Pan Wei-Ya Lang Li-Jie Yao Yan Wang Xiao-Ling Li | 2019 | World Journal of Gastrointestinal Oncology2019，11，8： | 6 |
| 14 | Recombinant vaccine containing an RBD-Fc fusion induced protection against SARS-CoV-2 in nonhuman primates and mice显示文摘The novel coronavirus SARS-CoV-2 has infected more than 104 million individuals and resulted in more than 2.2 million deaths worldwide as of February 7,2021(http://gffzze7c39cdc9e894ca6scxupocunov6q6vvu.ffgz.tsg.suse.edu.cn).The COVID-19 pandemic highlights the need for safe and effective vaccines against SARS-CoV-2 infection. | Shihui Sun Lei He Zhongpeng Zhao Hongjing Gu Xin Fang Tiecheng Wang Xiaolan Yang Shaolong Chen Yongqiang Deng Jiangfan Li Jian Zhao Liang Li Xinwang Li Peng He Ge Li Hao Li Yuee Zhao Chunrun Gao Xiaoling Lang Xin Wang Guoqiang Fei Yan Li Shusheng Geng Yuwei Gao Wenjin Wei Zhongyu Hu Gencheng Han Yansong Sun | 2021 | Cellular & Molecular Immunology2021，18，4： | 5 |
| 15 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 16 | Microstructural evolution of a PM TiAl alloy during heat treatment in α+γ phase field显示文摘In this study, the effect of temperatures and cooling rates of heat treatment on the microstructure of a powder metallurgy (PM) Ti-46Al-2Cr-2Nb-(B,W) (at.%) alloy was studied. Depending on the cooling rate and temperature, the different structures were obtained from the initial near-γ (NG) microstructures by heat treatment in the α+γ field. The results show that the microstructures of samples after furnace cooling (FC) consist primarily of equiaxed γ and α 2 grains, with a few grains containing lamellae. Duplex microstructures consist mainly of γ grains and lamellar colonies were obtained in the quenching into another furnace at 900°C (QFC) samples. However, further in- creasing of the cooling rate to air cooling (AC) induces the transformation of α→α_2 and results in a microstructure with equiaxed γ and α_2 grains, and no lamellar colonies are found. | SU Meike ZHENG Lijing LANG Zebao YAN Jie ZHANG Hu | 2012 | Rare Metals2012，31，5： | 5 |
| 17 | Effect of surface area and heteroatom of porous carbon materials on electrochemical capacitance in aqueous and organic electrolytes显示文摘A series of porous carbon materials with wide range of specific surface areas and different heteroatom contents had been prepared using polyaniline as carbon precursor and KOH as an activating agent. Effect of surface area and heteroatom of porous carbon materials on specific capacitance was investigated thoroughly in two typical aqueous KOH and organic 1-butyl-3- methylimidazolium tetrafluoroborate/acetonitirle electrolytes. The different trends of capacitance performance were observed in these two electrolytes. Electrochemical analyses suggested that the presence of faradaic interactions on heteroatom-enriched carbon materials in organic environment is less significant than that observed in aqueous electrolytes. Thus, in aqueous electrolyte, a balance between surface area and heteroatom content of activated porous carbon would be found to develop a supercapacitor with high energy density. In organic electrolyte, the capacitance performance of porous carbon is strongly dependent on the surface area. The results may be useful for the design of porous carbon-based supercapacitor with the desired capacitive performance in aqueous and organic electrolytes. | WANG RuTao LANG JunWei YAN XingBin | 2014 | Science China Chemistry2014，57，11： | 4 |
| 18 | 3D printing of high-performance micro-supercapacitors with patterned exfoliated graphene/carbon nanotube/silver nanowire electrodes显示文摘Micro-supercapacitors(MSCs)show great potential as on-chip energy storage devices for portable electronics.The major flaw of thin-film MSCs is their low energy density.To improve the energy density,thicker electrodes are required.However,the fabrication of MSCs with thick electrodes remains a challenge.In this work,a novel 3D printing method is employed to fabricate high-performance MSCs with interdigitated exfoliated graphene(EG)/carbon nanotube(CNT)/silver nanowire(Ag NW)electrodes.The nanowelding of Ag NW junction plays a critical role in the realization of 3D printing.To enhance the electrochemical performances of EG,phosphorus atoms are incorporated into the carbon framework with 1.7 at%.The areal capacitance of the 3D printed MSC is 21.6 mF cm^(-2)at a scan rate of 0.01 V s^(-1).The areal energy density of the MSC ranges from 0.5 to 2μWh cm^(-2)with a maximum power density of 2.5 mW cm^(-2). | LIU Lang LU JunYong LONG XinLin ZHOU Ren LIU YingQuan WU YiTing YAN KangWei | 2021 | Science China(Technological Sciences)2021，64，5： | 4 |
| 19 | Improving the performance of all-solid-state supercapacitors by modifying ionic liquid gel electrolytes with graphene nanosheets prepared by arc-discharge显示文摘Ionic liquid gel polymers have widely been used as the electrolytes in all-solid-state supercapacitors,but they suffer from low ionic conductivity and poor electrochemical performance.Arc discharge is a fast,low-cost and scalable method to prepare multi-layered graphene nanosheets,and as-made graphene nanosheets(denoted as ad-GNSs) with few defects,high electrical conductivity and high thermal stability should be favorable conductive additive materials.Here,a novel ionic liquid gel polymer electrolyte based on an ionic liquid(EMIMNTF_2) and an copolymer(P(VDF-HFP)) was modified by the addition of ad-GNSs as an ionic conducting promoter.This modified gel electrolyte shows excellent thermal stability up to 400℃ and a wide electrochemical window of 3 V.An all-solid-state supercapacitor based on commercial activated carbon was fabricated using this modified ionic liquid gel polymer electrolyte,which shows obviously improved electrochemical behaviors compared with those of the corresponding all-solid-state supercapacitor using pure ionic liquid gel polymer electrolyte.Specially,smaller internal resistance,higher specific capacitance,better rate performance and cycling stability are achieved.These results indicate that the ionic liquid gel polymers modified by ad-GNSs would be promising and suitable gel electrolytes for high performance all-solid-state electrochemical devices. | Min-Jie Shi Sheng-Zhong Kou Bao-Shou Shen Jun-Wei Lang Zhi Yang Xing-Bin Yan | 2014 | Chinese Chemical Letters2014，25，6： | 4 |
| 20 | Selection and characterization of the novel anti-human PD-1 FV78 antibody from a targeted epitope mammalian cell-displayed antibody library显示文摘Currently,display-based methods are well established and widely used in antibody engineering for affinity maturation and structural stability improvement.We obtained a novel anti-human programmed death 1(PD-1)antibody using computer-aided design and a mammalian cell display technology platform.We used computer-aided modeling and distance geometry methods to predict and assign the key residues that contributed to the binding of human PD-L1 to PD-1.Then,we analyzed the sequence of nivolumab(an anti-human PD-1 antibody,referred to as MIL75 in the article)to determine the template for antibody design and library construction.We identified a series of potential substitutions on the obtained template and constructed a virtual epitope-targeted antibody library based on the physicochemical properties and each possible location of the assigned key residues.The virtual antibody libraries were displayed on the surface of mammalian cells as the antigen-binding fragments of full-length immunoglobulin G.Then,we used flow cytometry and sequencing approaches to sort and screen the candidates.Finally,we obtained a novel anti-human PD-1 antibody named FV78.FV78 competitively recognized the PD-1 epitopes that interacted with MIL75 and possessed an affinity comparable to MIL75.Our results implied that FV78 possessed equivalent bioactivity in vitro and in vivo compared with MIL75,which highlighted the probability and prospect of FV78 becoming a new potential antibody therapy. | Longlong Luo Shi Wang Xiaoling Lang Tingting Zhou Jing Geng Xinying Li Chunxia Qiao Jiannan Feng Beifen Shen Ming Lv Yan Li | 2018 | Cellular & Molecular Immunology2018，15，2： | 4 |
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